cayman.700480 Glycogen Assay Kit 96T/盒Cayman


货品编号: cayman.700480 品牌:cayman 品名:Glycogen Assay Kit 规格:96T/盒

产品描述: Features Measure glycogen from tissue samples Assay 20 samples in duplicate Assay Range: 2.5-40 µg/ml Plate-based fluorometric measurement (ex 530-540 nm, em 585-595 nm)

Glycogen is a polysaccharide that is the principal storage form of glucose in animal and human cells. Glycogen is made

primarily by the liver and muscles, but it can also be made by glycogenesis within the brain and stomach. Cayman’s Glycogen

Assay provides a simple, reproducible, and sensitive tool for assaying glycogen from tissue. In this assay, glycogen is hydrolyzed

by amyloglucosidase to form β-D-glucose, which is then specifically oxidized to D-glucono-δ-lactone by glucose oxidase forming

hydrogen peroxide in the process. Hydrogen peroxide, in the presence of horseradish peroxidase, reacts with 10-acetyl-3,

7-dihydroxyphenoxazine (ADHP) in a 1:1 stoichiometry to generate the highly fluorescent product resorufin which is measure at

an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm.

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