货品编号： cst.2461s

品牌： cst

品名： Phospho-Rac1/cdc42 (Ser71) Antibody

规格： 100ul

产品描述：

Rac and Cdc42 are members of the Rho-GTPase family. In mammals, Rac exists as three isoforms, Rac1,

Rac2 and Rac3, which are highly similar in sequence. Rac1 and Cdc42, the most widely studied of this

group, are ubiquitously expressed. Rac2 is expressed in cells of hematopoietic origin, and Rac3, while

highly expressed in brain, is also found in many other tissues. Rac and Cdc42 play key signaling roles in

cytoskeletal reorganization, membrane trafficking, transcriptional regulation, cell growth and development .

GTP binding stimulates the activity of Rac/Cdc42, and the hydrolysis of GTP to GDP through the protein's

intrinsic GTPase activity, rendering it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs),

while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs). Another level

of regulation is achieved through the binding of RhoGDI, a guanine nucleotide dissociation inhibitor, which

retains Rho family GTPases, including Rac and Cdc42, in their inactive GDP-bound state (2,3).

A putative Akt phosphorylation site at Ser71 of Rac1/cdc42 has been identified and confirmed by in vitro kinase

assay . Phosphorylation at this site may inhibit GTP binding of Rac1, attenuating the signal transduction pathway

downstream of Rac1 .