货品编号: Invitrogen.A32723
品牌: Invitrogen
品名: Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488
规格: 1MG
产品描述:
To minimize cross-reactivity, the goat anti-mouse IgG whole antibodies have been pre cross-adsorbed against bovine IgG,
goat IgG, rabbit IgG, rat IgG, human IgG, and human serum. Cross-adsorption or pre-adsorption is a purification step to
increase specificity of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution
is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the
nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further
passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these
extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary
antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.
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