Invitrogen.A32731 Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 1MGInvitrogen


货品编号: Invitrogen.A32731

品牌: Invitrogen

品名: Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488

规格: 1MG

产品描述:

To minimize cross-reactivity, the goat anti-rabbit IgG whole antibodies have been pre cross-adsorbed against bovine IgG,

goat IgG, mouse IgG, rat IgG, and human IgG. Cross-adsorption or pre-adsorption is a purification step to increase specificity

of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution is passed through a

column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding

secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through

additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are

apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or

in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.

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