品牌： gibco

品名： RPMI 1640 Medium, GlutaMAX™, HEPES

规格： 500 mL

产品描述：

RPMI 1640 Medium (Roswell Park Memorial Institute 1640 medium) was originally developed to culture human leukemic

cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells,

including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 modifications

for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI is modified as follows:With

• GlutaMAX™

• Phenol Red

• HEPES

The complete formulation is available.

Using RPMI 1640 Medium

RPMI 1640 medium is unique from other media because it contains the reducing agent glutathione and high concentrations

of vitamins. RPMI 1640 medium contains biotin, vitamin B12 and PABA, which are not found in Eagle's Minimal Essential

Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high

concentrations. RPMI 1640 medium with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell

viability and growth in an easy-to-use format. RPMI 1640 medium contains no proteins, lipids, or growth factors. Therefore,

RPMI 1640 medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 medium uses a

sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.