品牌： Thermo

品名： Biotin DecaLabel DNA Labeling Kit

规格： 30 reactions

产品描述：

Thermo Scientific Biotin DecaLabel DNA Labeling Kit is an advanced system for the efficient synthesis of biotin-labeled

DNA probes, based on an improved random-primed labeling method developed by Feinberg and Vogelstein. The primary

improvement over traditional random-primed method involves the use of random decamers instead of hexamers, ensuring

more efficient annealing with DNA at 37°C. Klenow Fragment, exo-, included in the kit, is genetically engineered enzyme

with no detectable exonuclease activity. The enzyme does not degrade the labeled probe during reaction, which results in

a high labeling yield even with low amounts of template. You can uniformly label any length DNA fragments.

Biotin-labeled DNA is detected with the Biotin Chromogenic Detection Kit or conventional biotin-avidin or biotin-streptavidin

detection systems.

Highlights

• Non-radioactive labeling of DNA

• Efficient priming of labeling reactions with random decamers

• High yields with Klenow Fragment, exo-: no degradation of a labeled probe during reaction

Applications

• Generation of biotin-labeled DNA probes for a variety of non-radioactive hybridization experiments, including Southern and

Northern blots, colony/plaque hybridizations, dot/slot blots, and in situ hybridizations.