货品编号Bioss.bs-0573R

品牌Bioss

品名Anti-Cyclin E1周期素E抗体

规格100ul

研究领域细胞生物 细胞周期蛋白

抗体来源Rabbit

克隆类型Polyclonal

交叉反应Human, Mouse, Rat,

产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量45kDa

细胞定位细胞核

性 状Liquid

浓 度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from rat Cyclin E:375-411/411

亚 型IgG

纯化方法affinity purified by Protein A

储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

PubMedPubMed

产品介绍The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are

characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function

as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns

which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with

and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition.

This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase.

Overexpression of this gene has been observed in many tumors, which results in chromosome instability,

and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in,

the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in

cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression

in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct

isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence

information is not yet available. Transcript Variant: This variant (1) contains a different 5' end region, which

includes an upstream in-frame translation start codon, when compared to variant 2. The encoded protein has a

15 aa longer N-terminus, as compared to isoform 2.