货品编号： Bioss.bs-5194R 品 牌： Bioss 品 名：Anti-phospho-AKT1 (Thr34)磷酸化蛋白激酶B抗体 规 格： 100ul 产品类型 磷酸化抗体 研究领域 肿瘤免疫学信号转导细胞凋亡转录调节因子激酶和磷酸酶 抗体来源 Rabbit 克隆类型 Polyclonal 交叉反应 Human,Mouse,Rat, 产品应用 WB=1:500-2000ELISA=1:500-1000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=2μg/TestICC=1:100IF=1:100-500（石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. 分子量 56kDa 细胞定位 细胞核细胞浆细胞膜 性状 Liquid 浓度 1mg/ml 免疫原 KLH conjugated Synthesised phosphopeptide derived from human AKT1 around the phosphorylation site of Thr34:DG(p-T)FI 亚型 IgG 纯化方法 affinity purified by Protein A 储存液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. 保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. PubMed PubMed

产品介绍 The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized

fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is

abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol

3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can

suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and

inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively

spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]