货品编号Bioss.bs-0876R

品牌Bioss

品名Anti-phospho-AKT (Ser473)磷酸化蛋白激酶B抗体

规格100ul

抗体来源Rabbit

克隆类型Polyclonal

交叉反应Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Sheep,

产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test IF=1:100-500 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量56kDa

细胞定位细胞核 细胞浆 细胞膜

性 状Liquid

浓 度1mg/ml

免 疫 原KLH conjugated Synthesised phosphopeptide derived from human AKT around the phosphorylation site of Ser473:QF(p-S)YS

亚 型IgG

纯化方法affinity purified by Protein A

储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

PubMedPubMed

产品介绍The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved

primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor.

The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1.

It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system

AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in

a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates

and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the

Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.