货品编号Bioss.bs-1287R

品牌Bioss

品名Anti-IKB alpha核因子κB抑制蛋白α抗体

规格100ul

抗体来源Rabbit

克隆类型Polyclonal

交叉反应Human, Mouse, Rat,

产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量36kDa

细胞定位细胞核 细胞浆

性 状Liquid

浓 度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from human IKB alpha:1-120/314

亚 型IgG

纯化方法affinity purified by Protein A

储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

PubMedPubMed

产品介绍Three major forms of IKB like molecules have been identified and each is characterised by multiple copies of

ankyrin repeats. IKB alpha and IKB beta appear to be the major regulatory forms of IKB in most cells. These

proteins interact with p65 or cRel containing forms of NFkB and block nuclear import by masking the nuclear

localisation sequences of NFkB. The activation of NFkB involves the inducible phosphorylation and subsequent

degradation of IKB. Immunoblotting easily detects the hyperphosphorylated forms of IKB alpha, but not

phosphorylated IKB beta. Interestingly, IKB alpha and IKB beta mediate different NFkB responses. IkB alpha

appears to control more transient activation of NFkB in response to an inducer, while IKB beta controls a persistent

response. Bcl3 interacts with p50 and p52 containing forms of NFkB, but rather than being an inhibitor it appears

to function to stimulate transcription. The degradation of IKB is confirmed by immunoblotting.