货品编号： Bioss.bsm-33207M 品 牌： Bioss 品 名：Anti-TNF alpha肿瘤坏死因子-α单克隆抗体 规 格： 100ul 研究领域 细胞骨架细胞外基质 抗体来源 Mouse 克隆类型 Monoclonal 克隆号 3A4 交叉反应 Mouse,Rat,Chicken,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,Monkey,Insect, Yeast 产品应用 WB=1:1000-2000ELISA=1:5000-10000IHC-P=1:200-500IHC-F=1:200-500ICC=1:100-500IF=1:200-500（石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. 分子量 17/26kDa 细胞定位 细胞膜分泌型蛋白 性状 Liquid 浓度 1mg/ml 免疫原 Recombinant human TNF alpha 77-233aa : 亚型 IgG1 纯化方法 affinity purified by Protein G 储存液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. 保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

PubMed PubMed

产品介绍 Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis

when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia.

TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under

non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same

receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma

in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial

cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils

and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment

of certain cancers and AIDS Related Complex.