货品编号： InvitrogenA10040 品 牌：Invitrogen 品 名：Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 546 规 格： 1mg 产品描述： These donkey anti-rabbit IgG (H+L) whole secondary antibodies have been affinity-purified and show minimum cross-reactivity to bovine,

chicken, goat, guinea pig, hamster, horse, human, mouse, rat, and sheep serum proteins. Cross-adsorption or pre-adsorption is a purification

step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is

passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding

secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent

in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in

tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.

Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 546 dye is a bright, orange-fluorescent

dye with excitation ideally suited to the 546 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 546 dye is pH-

insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance

biological structures with great sensitivity. Alexa Fluor 546 dye molecules can be attached to proteins at high molar ratios without significant self-

quenching, enabling brighter conjugates and more sensitive detection. The degree of labeling for each conjugate is typically 2-8 fluorophore

molecules per IgG molecule; the exact degree of labeling is indicated on the certificate of analysis for each product lot.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment.

This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because

staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. For the fluorophore-labeled antibodies a

final concentration of 1-10 µg/mL should be satisfactory for most immunohistochemistry and flow cytometry applications.