货品编号： InvitrogenA31573 品 牌：Invitrogen 品 名：Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 规 格： 1mg 产品描述： To minimize cross-reactivity, these donkey anti-rabbit IgG whole antibodies have been affinity-purified and show a published cross-reactivity to

rat IgG. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less

background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially

cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow

through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential

cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous

immunoglobulins.

Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 647 dye is a near-infrared-fluorescent dye

with excitation ideally suited to the 647 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 647 dye is pH-insensitive

over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures

with great sensitivity. Alexa Fluor 647 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter

conjugates and more sensitive detection. The degree of labeling for each conjugate is typically 2-8 fluorophore molecules per IgG molecule; the exact

degree of labeling is indicated on the certificate of analysis for each product lot.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment.

This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because

staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. For the fluorophore-labeled antibodies a

final concentration of 1-10 µg/mL should be satisfactory for most immunohistochemistry and flow cytometry applications.