货品编号： abcam.ab65342 品 牌：abcam 品 名：Pyruvate Assay试剂盒(ab65342) 规 格： 100T 产品描述： Product name Pyruvate Assay Kit

Detection method Colorimetric/Fluorometric

Sample type Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Tissue Extracts

Assay type Quantitative

Sensitivity > 1 µM

Range 1 µM - 200 µM

Assay time 0h 40m

Product overview Pyruvate Assay Kit (Colorimetric/Fluorometric) ab65342 provides a simple, direct and automation-ready procedure for measuring

pyruvate concentration in various biological samples such as blood, cells, culture and fermentation media, etc.

In the pyruvate assay protocol, pyruvate is oxidized by pyruvate oxidase via enzyme reactions to generate color (λ= 570 nm) and

fluorescence (at Ex/Em = 535/587 nm). Since the color or fluorescence intensity is proportional to pyruvate content, the pyruvate

concentration can be accurately measured.

The kit detects 1-200 µM pyruvate.

Pyruvate assay protocol summary: - add samples and standards to wells - add reaction mix and incubate for30 min at room temp - analyze with microplate reader