Abcam.ab113851 DCFDA / H2DCFDA - Cellular ROS Assay Kit, 300 tests Abcam-耗材-金山科研平台 Thermo Fisher官网Nunc、QSP、Nalgene、Invitrogen、Gibco耗材试剂授权一级代理

Abcam.ab113851 DCFDA / H2DCFDA - Cellular ROS Assay Kit, 300 tests Abcam

货品编号： Abcam.ab113851 品牌：Abcam 品名：DCFDA / H2DCFDA - Cellular ROS Assay Kit, 规格： 300 tests 产品描述： Product name DCFDA / H2DCFDA- Cellular ROS Assay Kit See all Oxidative Stress kits

Detection method Fluorescent

Sample type Adherent cells, Suspension cells

Assay type Cell-based (quantitative)

Assay time 0h 40m

Product overview DCFDA - Cellular ROS Assay Kit / Reactive Oxygen Species Assay Kit (ab113851) uses the cell permeant reagent 2’,7’ –dichlorofluorescin

diacetate (DCFDA, also known asH2DCFDA, DCFH-DA, and DCFH).

DCFDA / H2DCFDA / DCFH-DA / DCFH is a fluorogenic dye that measures hydroxyl, peroxyl and other reactive oxygen species (ROS) activity

within the cell.

The DCFDA assay protocol is based on the diffusion of DCFDA / H2DCFDA / DCFH-DA/ DCFH into the cell. It is then deacetylated by cellular

esterases to a non-fluorescent compound, which is later oxidized by ROS into 2’, 7’ –dichlorofluorescein (DCF). DCF is highly fluorescent and

is detected by fluorescence spectroscopy with excitation / emission at485 nm / 535 nm.

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Abcam.ab113851 DCFDA / H2DCFDA - Cellular ROS Assay Kit, 300 tests Abcam

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