货品编号： Abcam.ab113851 品 牌：Abcam 品 名：DCFDA / H2DCFDA - Cellular ROS Assay Kit, 规 格： 300 tests 产品描述： Product name DCFDA / H2DCFDA- Cellular ROS Assay Kit See all Oxidative Stress kits
Detection method Fluorescent
Sample type Adherent cells, Suspension cells
Assay type Cell-based (quantitative)
Assay time 0h 40m
Product overview DCFDA - Cellular ROS Assay Kit / Reactive Oxygen Species Assay Kit (ab113851) uses the cell permeant reagent 2’,7’ –dichlorofluorescin
diacetate (DCFDA, also known asH2DCFDA, DCFH-DA, and DCFH).
DCFDA / H2DCFDA / DCFH-DA / DCFH is a fluorogenic dye that measures hydroxyl, peroxyl and other reactive oxygen species (ROS) activity
within the cell.
The DCFDA assay protocol is based on the diffusion of DCFDA / H2DCFDA / DCFH-DA/ DCFH into the cell. It is then deacetylated by cellular
esterases to a non-fluorescent compound, which is later oxidized by ROS into 2’, 7’ –dichlorofluorescein (DCF). DCF is highly fluorescent and
is detected by fluorescence spectroscopy with excitation / emission at485 nm / 535 nm.
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