博士德.A04154-2 Anti-NFIC Antibody 50ul博士德


货品编号: 博士德.A04154-2

品牌: 博士德

品名: Anti-NFIC Antibody

规格: 50ul

货号A04154-2
产品名称Anti-NFIC Antibody
基因名NFIC
抗体来源Rabbit
克隆Polyclonal
抗体亚型Rabbit IgG
分子量55-65KD
免疫原E.coli-derived human NFIC/CTF recombinant protein (Position: D215-G508).
内容500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式Immunogen affinity purified.
浓度500 ug/ml
产品形态Liquid
保存条件12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料Nuclear factor 1 C-type is a protein that in humans is encoded by the NFIC gene. It is mapped to 19p13.3. The protein encoded by this gene belongs to the CTF/NF-I family. These are dimeric DNA-binding proteins, and function as cellular transcription factors and as replication factors for adenovirus DNA replication. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
研究类别1. Grunder, A., Qian, F., Ebel, T. T., Mincheva, A., Lichter, P., Kruse, U., Sippel, A. E. Genomic organization, splice products and mouse chromosomal localization of genes for transcription factor nuclear factor one. Gene 304: 171-181, 2003.2. Qian, F., Kruse, U., Lichter, P., Sippel, A. E. Chromosomal localization of the four genes (NFIA, B, C, and X) for the human transcription factor nuclear factor I by FISH. Genomics 28: 66-73, 1995.3. Steele-Perkins, G., Butz, K. G., Lyons, G. E., Zeichner-David, M., Kim, H.-J., Cho, M.-I., Gronostajski, R. M. Essential role for NFI-C/CTF transcription-replication factor in tooth root development. Molec. Cell. Biol. 23: 1075-1084, 2003.
Uniprot IDNFIC:P08651
推荐配套的二抗和检测试剂Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
产品应用细节

为了提供最优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。

应用稀释度*
Western blot:1:500-2000
Immunohistochemistry(Paraffin-embedded Section):1:50-400
Immunocytochemistry/Immunofluorescence (ICC/IF):1:50-400
Flow Cytometry:1-3 μg/1x106cells
Direct ELISA:1:100-1000

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