Bioss.bs-4843R-50ul Anti-LC3B 自噬微管相关蛋白轻链β3抗体Bioss-耗材-金山科研平台 Thermo Fisher官网Nunc、QSP、Nalgene、Invitrogen、Gibco耗材试剂授权一级代理

Bioss.bs-4843R-50ul Anti-LC3B 自噬微管相关蛋白轻链β3抗体Bioss

货品编号： Bioss.bs-4843R-50ul

品牌： Bioss

品名： Anti-LC3B自噬微管相关蛋白轻链β3抗体

规格： 50ul

产品介绍

Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and in maintaining the balance between neuronal plasticity and rigidity. MAP-light chain 3 beta (MAP-LC3 Beta) and MAP-light chain 3 alpha (MAP-LC3 Alpha) are subunits of both MAP1A and MAP1B. MAP-LC3 Beta, a homolog of Apg8p, is essential for autophagy and associated to the autophagosome membranes after processing. Two forms of LC3 Beta, the cytosolic LC3-I and the membrane-bound LC3-II, are produced post-translationally. LC3-I is formed by the removal of the C-terminal 22 amino acids from newly synthesized LC3 Beta, followed by the conversion of a fraction of LC3-I into LC3-II. LC3 enhances fibronectin mRNA translation in ductus arteriosus cells through association with 60S ribosomes and binding to an AU-rich element in the 3’ untranslated region of fibronectin mRNA. This facilitates sorting of fibronectin mRNA onto rough endoplasmic reticulum and translation. MAP LC3 Beta may also be involved in formation of autophagosomal vacuoles. It is expressed primarily in heart, testis, brain and skeletal muscle.Function:Probably involved in formation of autophagosomal vacuolesSubunit:3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins. Interacts at microtubules with CABP1 (via EF-hands 1 and 2) but not with calmodulin. Interacts with FYCO1 (via C-terminus). Interacts with TP53INP1 and TP53INP2. Interacts with TBC1D25. Directly interacts with SQSTM1; this interaction leads to MAP1LC3B recruitment to inclusion bodies containing polyubiquitinated protein aggregates and to inclusion body degradation by autophagy.Subcellular Location:Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note=LC3-II binds to the autophagic membranes.Tissue Specificity:Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.Post-translational modifications:The precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II.Similarity:Belongs to the MAP1 LC3 family.SWISS:Q9GZQ8Gene ID:81631Database links:

Entrez Gene: 81631Human

Entrez Gene: 427559Chicken

Entrez Gene: 408001Cow

Entrez Gene: 67443Mouse

Entrez Gene: 64862Rat

Omim: 609604Human

SwissProt: O41515Cow

SwissProt: Q9GZQ8Human

SwissProt: Q9CQV6Mouse

SwissProt: Q62625Rat

Unigene: 356061Human

Unigene: 28357Mouse

Unigene: 41412Rat

Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片

Sample:Lane 1: Cerebrum (Rat) Lysate at 40 ugLane 2: SH-SY5Y (Human) Cell Lysate at 30 ugLane 3: Hela (Human) Cell Lysate at 30 ugLane 4: HepG2 (Human) Cell Lysate at 30 ugPrimary: Anti-LC3B (bs-4843R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 14/17 kDObserved band size: 17 kDBlank control(blue): TM4 cells(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice).Primary Antibody:Rabbit Anti-LC3B antibody(bs-4843R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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