MIP-3β是一种CC趋化因子，在胸腺，淋巴结和活化的骨髓基质细胞中表达，通过CCR7受体传导。 MIP-3β是T和B淋巴细胞和骨髓祖细胞的趋化因子。
BioGems人MIP-3β（CCL19）预包被ELISA（酶联免疫吸附测定）试剂盒采用固相免疫法，专门设计用于检测MIP-3β（CCL19），96孔微板预包被MIP-3β特异鼠单克隆抗体（CCL19）。它含有大肠杆菌表达的重组MIP-3β（CCL19）。检测抗体是MIP-3β特异生物素化山羊多克隆抗体（CCL19）。该试剂盒是即用型试剂盒，已经过分析验证。
Human MIP-3β (CCL19) Pre-Coated ELISA Kit分析
Product Details
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Assay Type:
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Solid Phase Sandwich ELISA: 96-Well Strip Plate
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Sensitivity:
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<10pg/ml
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Range:
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15.6pg/ml-1000pg/ml
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Specificity:
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Natural and recombinant Human MIP-3β(CCL19)
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|
Uniprot:
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Q6IBD6
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|
Interference:
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No detectable cross-reactivity with other relevant proteins
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Storage Conditions:
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Store the unopened product at 2 – 8 ℃.
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Components组份
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Description
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Quantity
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Volume
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Anti-Human MIP-3β(CCL19) Pre-coated 96-well strip microplate
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1
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12 strips of 8 wells
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Human MIP-3β(CCL19) Standard
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2
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10 ng vial
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Human MIP-3β(CCL19) Biotinylated antibody (100x)
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1
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130 µL
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Avidin-Biotin-Peroxidase Complex (100x)
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1
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130 µL
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Sample Diluent
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1
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30mL
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Antibody Diluent
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1
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12mL
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Avidin-Biotin-Peroxidase Diluent
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1
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12mL
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Wash Buffer Concentrate (Powder for 1000mL)
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1
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Pack
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Color Developing Reagent
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1
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10mL
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Stop Solution
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1
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10mL
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Plate Sealers
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4
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Each
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Reproducibility可重复性
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|
Intra-Assay
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Inter-Assay
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|
Sample ID
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1
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2
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3
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1
|
2
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3
|
|
n=
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16
|
16
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16
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24
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24
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24
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Mean Measured Concentration (pg/mL)
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97
|
293
|
583
|
117
|
374
|
627
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Standard Deviation
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5.53
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14.1
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36.15
|
7.5
|
18.7
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42.64
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Consistency (%CV)
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5.7
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4.8
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6.2
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6.4
|
5
|
6.8
|
Catalog Number : BGK6IBD6
Description
MIP-3β is a CC chemokine, expressed in the thymus, lymph nodes, and in activated bone marrow stromal cells that signals through the CCR7 receptor. MIP-3βis a chemoattractant for T and B lymphocytes, and myeloid progenitor cells.
The BioGems Human MIP-3β(CCL19) Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure MIP-3β(CCL19) with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for MIP-3β(CCL19). It contains E. coli-expressed recombinant MIP-3β(CCL19). The detection antibody is a biotinylated goat polyclonal antibody specific for MIP-3β(CCL19). The kit is analytically validated with ready to use reagents.
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