上皮调节蛋白是与EGFR（ErbB1）和ErbB4特异性结合但不结合ErbB2或ErbB3的EGF相关生长因子。 它主要表达于胎盘和外周血白细胞，以及膀胱，肺，肾和结肠的某些癌症中。上皮调节蛋白刺激角质形成细胞，肝细胞，成纤维细胞和血管平滑肌细胞的增殖。它还抑制几种肿瘤来源的上皮细胞系的生长。
BioGems Human Epiregulin Pre-Coated ELISA（酶联免疫吸附测定）试剂盒是采用固相免疫测定法，专门设计用于检测Epiregulin，96孔微板上预包被Epiregulin特异性小鼠单克隆抗体。 它含有大肠杆菌表达的重组Epiregulin。 检测抗体是对Epiregulin特异的生物素化山羊多克隆抗体。该试剂盒已经过分析验证，是即用型试剂。
Product Details
|
Assay Type:
|
Solid Phase Sandwich ELISA: 96-Well Strip Plate
|
|
Sensitivity:
|
<10pg/ml
|
|
Range:
|
156pg/ml-10,000pg/ml
|
|
Specificity:
|
Natural and recombinant Human Epiregulin
|
|
Uniprot:
|
O14944
|
|
Interference:
|
No detectable cross-reactivity with other relevant proteins
|
|
Storage Conditions:
|
Store the unopened product at 2 – 8 ℃.
|
Components
|
Description
|
Quantity
|
Volume
|
|
Anti-Human Epiregulin Pre-coated 96-well strip microplate
|
1
|
12 strips of 8 wells
|
|
Human Epiregulin Standard
|
2
|
10 ng vial
|
|
Human Epiregulin Biotinylated antibody (100x)
|
1
|
130µL
|
|
Avidin-Biotin-Peroxidase Complex (100x)
|
1
|
130 µL
|
|
Sample Diluent
|
1
|
30mL
|
|
Antibody Diluent
|
1
|
12mL
|
|
Avidin-Biotin-Peroxidase Diluent
|
1
|
12mL
|
|
Wash Buffer Concentrate (Powder for 1000mL)
|
1
|
Pack
|
|
Color Developing Reagent
|
1
|
10mL
|
|
Stop Solution
|
1
|
10mL
|
|
Plate Sealers
|
4
|
Each
|
Reproducibility
|
|
Intra-Assay
|
Inter-Assay
|
|
Sample ID
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n=
|
16
|
16
|
16
|
24
|
24
|
24
|
|
Mean Measured Concentration (pg/mL)
|
1.15
|
2.52
|
5.81
|
1.63
|
2.87
|
5.66
|
|
Standard Deviation
|
0.059
|
0.113
|
0.273
|
0.096
|
0.146
|
0.35
|
|
Consistency (%CV)
|
5.2
|
4.5
|
4.7
|
5.9
|
5.1
|
6.2
|
Description
Epiregulin is an EGF-related growth factor that binds specifically to EGFR (ErbB1) and ErbB4, but not ErbB2 or ErbB3. It is expressed mainly in the placenta and peripheral blood leukocytes, as well as in certain carcinomas of the bladder, lung, kidney and colon. Epiregulin stimulates the proliferation of keratinocytes, hepatocytes, fibroblasts and vascular smooth muscle cells. It also inhibits the growth of several tumor-derived epithelial cell lines.
The BioGems Human Epiregulin Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Epiregulin with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for Epiregulin. It contains E. coli-expressed recombinant Epiregulin. The detection antibody is a biotinylated goat polyclonal antibody specific for Epiregulin. The kit is analytically validated with ready to use reagents.
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