代理BioGems 人Follistatin样蛋白1预包被ELISA试剂盒,Human FSTL1 Pre-Coated ELISA kit 现货


Follistatin样蛋白1(FSTL1)是一种广泛表达的细胞外糖蛋白,homologously grouped into the osteonectin (BM-40/SPARC) family of secreted proteins based on its possession of both a follistatin-like and extracellular calcium-binding domain。FSTL1最初在克隆的小鼠成骨细胞系中鉴定为TGF-β诱导型蛋白质,然后发现FSTL1参与一系列cell-type-specific功能,例如细胞扩增,分化,凋亡和迁移调节,以及许多生物过程,包括胚胎发育,炎症反应,血管生成,肿瘤发生和免疫疾病发病机制。FSTL1Highly conserved across mammalian species并在人体组织中广泛表达,可以通过先天免疫系统的信号传递介质(例如TLR4激动剂和通过NFκB途径的关节炎发生细胞因子IL-1β)上调,以刺激 pro-inflammatory cytokines(包括TNF-α,IL-1β,IL-6和IL-8)表达和分泌。尽管间充质谱系的细胞能够产生FSTL1,但在正常生理条件下,造血谱系细胞中明显不存在FSTL1表达。然而,巨噬细胞和单核细胞能够在炎症部位吸收FSTL1,其中FSTL1刺激可以引起胱天蛋白酶-1的表达及其从pro-IL-1β酶切active IL-1β。尽管已经指出FSTL1的过表达已经被认为是免疫疾病如类风湿性关节炎(RA)和骨关节炎(OA)实质进展因素,但是FSTL1血清水平的降低已经被确定为在卵巢癌和子宫内膜癌发生中起重要作用,其中它直接影响细胞增殖,迁移和入侵。

BioGems人类FSTL1预包被ELISA(酶联免疫吸附测定)试剂盒是采用固相免疫测定法,专门设计用于测量FSTL1,96孔板预包被有FSTL1特异性的小鼠单克隆抗体。它含有NS0表达的重组FSTL1。检测抗体是对FSTL1特异性的生物素化山羊多克隆抗体。该试剂盒已经过分析验证,是即用型试剂。

Product Details

Assay Type:

Solid Phase Sandwich ELISA: 96-Well Strip Plate

Sensitivity:

<10pg/ml

Range:

312pg/ml-20,000pg/ml

Specificity:

Natural and recombinant Human FSTL1

Uniprot:

Q12841

Interference:

No detectable cross-reactivity with other relevant proteins

Storage Conditions:

Store the unopened product at 2 – 8 ℃.

Components

Description

Quantity

Volume

Anti-Human FSTL1 Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Human FSTL1 Standard

2

10 ng vial

Human FSTL1 Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

1.34

7.2

13.9

1.25

7.5

14.4

Standard Deviation

0.056

0.382

0.89

0.094

0.48

1.123

Consistency (%CV)

4.2

5.3

6.4

7.5

6.4

7.8

Follistatin-like protein 1 (FSTL1) is a widely-expressed, extracellular glycoprotein that is homologously grouped into the osteonectin (BM-40/SPARC) family of secreted proteins based on its possession of both a follistatin-like and extracellular calcium-binding domain. Initially identified as a TGF-β-inducible protein in a cloned mouse osteoblast cell line, FSTL1 has since been implicated in an array of cell-type-specific functions, such as the regulation of proliferation, differentiation, apoptosis and migration, as well as a number of biological processes, including embryonic development, inflammatory response, angiogenesis, tumorigenesis, and immune disease pathogenesis. Highly conserved across mammalian species and widely expressed in human tissues, FSTL1 can be upregulated through signaling mediators of the innate immune system,such as TLR4 agonists and the arthritogenic cytokine IL-1β via NFκB pathways, to stimulate the expression and secretion of pro-inflammatory cytokines, including TNF-α, IL-1β, IL-6 and IL-8. While cells of mesenchymal lineage are capable of FSTL1 production, FSTL1 expression is notably absent from cells of hematopoietic lineage under normal physiological conditions. Macrophages and monocytes are, however, capable of taking up FSTL1 at sites of inflammation where FSTL1 stimulation can cause the expression of caspase-1 and its resultant enzymatic cleavage of active IL-1β from pro-IL-1β. Whereas the overexpression of FSTL1 has been noted as a substantial contributor to the progression of immune diseases like rheumatoid arthritis (RA) and osteoarthritis (OA), diminished FSTL1 serum levels have been identified as playing a significant part in both ovarian and endometrial carcinogenesis, where it directly affects cell proliferation, migration and invasion.

The BioGems Human FSTL1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure FSTL1 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for FSTL1. It contains NS0-expressed recombinant FSTL1. The detection antibody is a biotinylated goat polyclonal antibody specific for FSTL1. The kit is analytically validated with ready to use reagents.

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