神经调节蛋白/调蛋白是由选择性剪接基因(NRG1,NRG2,NRG3和NRG4)衍生的结构相关多肽生长因子家族。迄今为止,有超过14种来源于NRG1基因的可溶性和跨膜蛋白。跨膜NRG1同种型的胞外域的蛋白水解加工释放可溶性生长因子。 HRG1-β1含有Ig结构域和EGF样结构域;后者对于直接结合受体酪氨酸激酶erb3和erb4是必需的。该结合诱导erb3和erb4与erb2异源二聚化,刺激导致酪氨酸磷酸化的内在激酶活性。
BioGems人类神经调节蛋白预包被ELISA(酶联免疫吸附测定)试剂盒是一种固相免疫测定,专门设计用于测量Neuregulin的96孔带板,其预先包被有特异于神经调节蛋白的小鼠单克隆抗体。它含有大肠杆菌表达的重组Neuregulin。检测抗体是特异于Neuregulin的生物素化山羊多克隆抗体。该试剂盒已经过分析验证,是即用型试剂。
Product Details
Assay Type:
|
Solid Phase Sandwich ELISA: 96-Well Strip Plate
|
Sensitivity:
|
<10pg/ml
|
Range:
|
62.5pg/ml-4000pg/ml
|
Specificity:
|
Natural and recombinant Human Neuregulin
|
Uniprot:
|
Q02297
|
Interference:
|
No detectable cross-reactivity with other relevant proteins
|
Storage Conditions:
|
Store the unopened product at 2 – 8 ℃
|
Components
Description
|
Quantity
|
Volume
|
Anti-Human Neuregulin Pre-coated 96-well strip microplate
|
1
|
12 strips of 8 wells
|
Human Neuregulin Standard
|
2
|
10 ng vial
|
Human Neuregulin Biotinylated antibody (100x)
|
1
|
130µL
|
Avidin-Biotin-Peroxidase Complex (100x)
|
1
|
130 µL
|
Sample Diluent
|
1
|
30mL
|
Antibody Diluent
|
1
|
12mL
|
Avidin-Biotin-Peroxidase Diluent
|
1
|
12mL
|
Wash Buffer Concentrate (Powder for 1000mL)
|
1
|
Pack
|
Color Developing Reagent
|
1
|
10mL
|
Stop Solution
|
1
|
10mL
|
Plate Sealers
|
4
|
Each
|
Reproducibility
|
Intra-Assay
|
Inter-Assay
|
Sample ID
|
1
|
2
|
3
|
1
|
2
|
3
|
n=
|
16
|
16
|
16
|
24
|
24
|
24
|
Mean Measured Concentration (pg/mL)
|
675
|
1308
|
2147
|
694
|
1382
|
2503
|
Standard Deviation
|
28.35
|
61.48
|
113.8
|
40.25
|
84.3
|
172.7
|
Consistency (%CV)
|
4.2
|
4.7
|
5.3
|
5.8
|
6.1
|
6.9
|
Neuregulin/Heregulin is a family of structurally related polypeptide growth factors derived from alternatively spliced genes (NRG1, NRG2, NRG3 and NRG4). To date, there are over 14 soluble and transmembrane proteins derived from the NRG1 gene. Proteolytic processing of the extracellular domain of the transmembrane NRG1 isoforms releases soluble growth factors. HRG1-β1 contains an Ig domain and an EGF-like domain; the latter is necessary for direct binding to receptor tyrosine kinases erb3 and erb4. This binding induces erb3 and erb4 heterodimerization with erb2, stimulating intrinsic kinase activity that leads to tyrosine phosphorylation.
The BioGems Human Neuregulin Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Neuregulin with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for Neuregulin. It contains E. coli-expressed recombinant Neuregulin. The detection antibody is a biotinylated goat polyclonal antibody specific for Neuregulin. The kit is analytically validated with ready to use reagents.
©
金山科研平台 Thermo Fisher官网Nunc、QSP、Nalgene、Invitrogen、Gibco耗材试剂授权一级代理是专业的授权总代理区域代理经销平台。
© 如需询价,请加客服QQ:1749072012 、客服微信:jinshanbio,或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务,数据资源基于CC协议。
© 本文地址:
https://thermonunc.cn/thread-19914.htm
特别声明:以上内容(如有图片亦包括在内)来源于授权厂家或网络,如有侵权,请联系客服删除,本平台不提供信息校正和存储服务。
Notice: The content above (including the pictures if any)comes from authorized manufacturers or networks. In case of infringement, please contact to delete it. This platform does not provide information storage services.