RANTES是CC趋化因子，可通过CCR1，CCR3，CCR5和US28（巨细胞病毒受体）受体传导信号。 它是一种趋化单核细胞，记忆T细胞（CD4 + / CD45RO），嗜碱性粒细胞和嗜酸性粒细胞的趋化剂。RANTES还具有抑制某些HIV-1，HIV-2和猿免疫缺陷病毒（SIV）的能力。
BioGems人类RANTES预包被ELISA（酶联免疫吸附测定）试剂盒采用固相免疫测定法，专门设计用于检测RANTES，96孔板预包被RANTES特异性小鼠单克隆抗体。它含有大肠杆菌表达的重组RANTES。检测抗体是RANTES特异性的生物素化山羊多克隆抗体。该试剂盒已经过分析验证，是即用型试剂。
Product Details
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Assay Type:
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Solid Phase Sandwich ELISA: 96-Well Strip Plate
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Sensitivity:
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<3pg/ml
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Range:
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15.6pg/ml-1000pg/ml
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Specificity:
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Natural and recombinant Human Rantes
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Uniprot:
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D0EI67
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|
Interference:
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No detectable cross-reactivity with other relevant proteins
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Storage Conditions:
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Store the unopened product at 2 – 8 ℃.
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Components
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Description
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Quantity
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Volume
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Anti-Human Rantes Pre-coated 96-well strip microplate
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1
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12 strips of 8 wells
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Human Rantes Standard
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2
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10 ng vial
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Human Rantes Biotinylated antibody (100x)
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1
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130 µL
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Avidin-Biotin-Peroxidase Complex (100x)
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1
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130 µL
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Sample Diluent
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1
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30mL
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Antibody Diluent
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1
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12mL
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Avidin-Biotin-Peroxidase Diluent
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1
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12mL
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Wash Buffer Concentrate (Powder for 1000mL)
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1
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Pack
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Color Developing Reagent
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1
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10mL
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Stop Solution
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1
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10mL
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Plate Sealers
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4
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Each
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Reproducibility
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|
Intra-Assay
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Inter-Assay
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|
Sample ID
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1
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2
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3
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1
|
2
|
3
|
|
n=
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16
|
16
|
16
|
24
|
24
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24
|
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Mean Measured Concentration (pg/mL)
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147
|
293
|
501
|
110
|
339
|
658
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Standard Deviation
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5.88
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15.53
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31.1
|
8.14
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24.41
|
56.6
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Consistency (%CV)
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4
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5.3
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6.2
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7.4
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7.2
|
8.6
|
Description
RANTES is a CC chemokine that can signal through the CCR1, CCR3, CCR5 and US28 (cytomegalovirus receptor) receptors. It is a chemoattractant towards monocytes, memory T cells (CD4+/CD45RO), basophils, and eosinophils. RANTES also has the capability to inhibit certain strains of HIV-1, HIV-2 and simian immunodeficiency virus (SIV).
The BioGems Human RANTES Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure RANTES with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for RANTES. It contains E. coli-expressed recombinant RANTES. The detection antibody is a biotinylated goat polyclonal antibody specific for RANTES. The kit is analytically validated with ready to use reagents.
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