Catalog Number :BGK27548

CD40, a member of the TNF receptor family, is a cell surface protein expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells. Signaling though CD40 plays an important role in the proliferation and differentiation of B cells, and is critical for immunoglobulin (Ig) class switching. The membrane-anchored CD40-Ligand is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to “immunodeficiency with hyper-IgM”, a disease characterized by failure to produce IgG, IgA and IgE.

The BioGems Murine CD40 Ligand Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is asolid phase immunoassay specially designed to measure CD40 Ligand with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for CD40 Ligand. It contains NS0-expressed recombinant CD40 Ligand. The detection antibody is a biotinylated goat polyclonal antibody specific for CD40 Ligand. The kit is analytically validated with ready to use reagents.

CD40是TNF受体家族的一员，是在B细胞，树突状细胞，单核细胞，胸腺上皮细胞上表达的细胞表面蛋白，在T细胞上表达水平低。CD40信号在B细胞的增殖和分化中起重要作用，并且对于免疫球蛋白（Ig）类型转换（class switching）起关键作用。膜锚定的CD40配体几乎完全只表达在活化的CD4 + T淋巴细胞上。未能表达CD40L会导致“高IgM免疫缺陷”，这是一种以未能产生IgG，IgA和IgE为特征的疾病。

BioGems鼠CD40配体预包被ELISA（酶联免疫吸附试验）试剂盒采用固相免疫测定法，专门设计用于检测CD40配体，并在96孔微孔板上预包被CD40配体特异性小鼠单克隆抗体。它含有NS0表达的重组CD40配体。检测抗体是CD40配体特异的生物素化山羊多克隆抗体。该试剂盒经过分析验证，是即用型试剂。

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