英文名称：Cellartis DEF-CS 500 Xeno-Free Culture Medium without Antibiotics

中文名称：Cellartis DEF-CS500无外源iPS培养基，不含抗生素

特点：不含抗生素，无饲养层，非酶法单细胞传代。

用途：临床前级别iPS细胞生产

保存：基础培养基2-8°C，添加剂-20°C

Cellartis DEF-CS 500 Xeno-Free Culture Medium 不含抗生素，适用于在完全不含人和动物来源组分的条件下扩增人诱导多能干（iPS）细胞。细胞不使用饲养层细胞的情况下培养；而是使用诸如iMatrix-511的无异源物基底。培养方案使用温和的非酶促单细胞传代以确保可控制性和重复性。Cellartis DEF-CS500无动物源培养基（不含抗生素）中培养的细胞具有高增殖率和稳定的核型，适合有效扩增。这些特性的结合使得该培养基成为再生医学研究临床前级别iPS细胞生产的理想选择。

特点：

不含人类或动物来源成分

优化用于培养临床前级人类iPS细胞

保留了iPS细胞的多能性和长期的自我更新和分化潜能

提供高重复性和稳定的生长

使用单细胞传代方法

组份：基础培养基+添加剂

Cellartis DEF-CS Xeno-Free Culture Medium without Antibiotics (Cat. No. Y30045)

500 ml Cellartis DEF-CS 500 Xeno-Free Basal Medium without Antibiotics (Cat. No Y30046; notsold separately)

Cellartis DEF-CS 500 Xeno-Free Additives (Cat. No. Y30042)

o 500 µl DEF-CS Xeno-Free Additive 1 (1000X)

o 200 µl DEF-CS Xeno-Free Additive 2 (1000X)

另行购买试剂：

缓冲液、培养基质和消化剂

PBS Dulbecco’s（含钙镁和不含钙镁）和细胞培养基质

Corning Synthemax II-SC Substrate (Corning, Cat. No. 3535) or iMatrix-511 (Takara Clontech, Cat. No. T303)

PBS Dulbecco’s with Ca2+ & Mg2+ (D-PBS +/+)

PBS Dulbecco’s w/o Ca2+ & Mg2+ (D-PBS –/–)

Versene Solution (Life Technologies, Cat. No. 15040)

Cellartis DEF-CS 500 Xeno-Free Culture Medium without Antibiotics is intended for the expansion of human induced pluripotent stem (iPS) cells in conditions that are completely free from human- and animal-derived components. Cells are cultured in the absence of feeder cells; instead, a xeno-free substrate such as iMatrix-511 is used. The culture protocol uses gentle, non-enzymatic single-cell passaging to ensure controlled and reproducible conditions. Cells cultured in Cellartis DEF-CS 500 Xeno-Free Culture Medium without Antibiotics have a high-proliferation rate and stable karyotype, allowing for efficient scale-up. The combination of these features makes this medium ideal for generating preclinical-grade iPS cells for regenerative medicine studies.