Cellartis DEF-CS500 iPS无外源3D悬浮培养基

传统的2D培养系统无法支持放大培养（Scale-up）所需细胞数量级别（> 109个细胞/处理）以维持细胞疗法的增长需求。3D悬浮培养是大规模生产多能细胞的可行经济有效的解决方案。尽管干细胞传统上难以适应悬浮培养，但CellArtis研究人员已经开发出一种培养体系，可以在成分确定、无饲养层和无异源物条件下实现稳定高效的生长并维持多能性。Cellartis DEF-CS500 iPS无外源3D悬浮培养基与生物反应器系统配套，为创建大量高质量，未分化的起始材料提供了理想的方法。该培养基的配方基于2D培养基Cellartis DEF-CS 500 Xeno-Free Culture Medium，2D培养的细胞可以轻松过渡到3D以实现可靠的放大。

英文名称：Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium w/o Antibiotics

中文名称：Cellartis DEF-CS500无外源3D悬浮iPS培养基，不含抗生素

用途：iPS细胞3D悬浮细胞治疗所需10亿级

保存：基础培养基2-8°C，添加剂-20°C

组份

基础培养基+三种添加剂

Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium w/o Antibiotics (Cat. No. Y30047)

500 ml Cellartis DEF-CS 500 Xeno-Free Basal Medium w/o Antibiotics (Cat. No Y30046; not soldseparately)

Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Additives (Cat. No. Y30048)

o 500 µl DEF-CS Xeno-Free 3D Spheroid Additive 1 (1,000X)

o 200 µl DEF-CS Xeno-Free 3D Spheroid Additive 2 (4,000X)

o 400 µl DEF-CS Xeno-Free 3D Spheroid Additive 3 (500X)

自行购买试剂

缓冲液和细胞分离试剂

PBS Dulbecco’s w/o Ca2+ & Mg2+ (D-PBS–/–)

Accumax (Merck Millipore, Cat. No. SCR006)

可选抗生素和消化试剂

Gentamicin (Thermo Fisher, Cat. No. 15750)

Versene Solution (Thermo Fisher, Cat. No. 15040) (for transfer from monolayer culture)

细胞培养参数

Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium

Traditional 2D culture systems simply cannot support the scale-up required (>109 cells/treatment) to sustain the growing demand for cell-based therapies. 3D suspension culture is a viable, cost-effective solution for large-scale production of pluripotent cells. Although stem cells have traditionally been difficult to adapt to suspension culture, our scientists have developed a culture system that allows robust, stable growth and maintenance of pluripotency under defined, feeder-free, and xeno-free conditions. The Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium paired with a perfusion bioreactor system provides an ideal method for creating large amounts of high-quality, undifferentiated starting material. The formulation of this medium is based on the 2D medium, Cellartis DEF-CS 500 Xeno-Free Culture Medium, and cells growing in 2D can easily be transitioned to 3D for robust scale-up.