货品编号： Thermofis.ER0201

品牌：Thermofis

品名： Cfr42I (SacII)

规格： 10 U/μL

产品描述

Thermo Scientific Cfr42I (SacII) restriction enzyme recognizes CCGC^GG sites and cuts best at 37°C in B buffer

(Isoschizomers: KspI, SacII, Sfr303I, SgrBI, SstII). See Reaction Conditions for Restriction Enzymes for a table of

enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes,

optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided

for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction

conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed

BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Features • Superior quality—stringent quality control and industry leading manufacturing process • Convenient color-coded Five Buffer System • Includes universal Tango buffer for double-digestions • BSA premixed in reaction buffers • Wide selection of restriction endonuclease specificities

Applications • Molecular cloning • Restriction site mapping • Genotyping • Southern blotting • Restriction fragment length polymorphism (RFLP) • SNP

Note: Cfr42I activity is affected by high salt concentration. Trace amounts of sodium chloride remaining in the substrate

DNA after completion of upstream applications may inhibit enzyme activity and result in impaired DNA cleavage. Certain

sites in lambda DNA are difficult to cleave with Cfr42I, the same as with its prototype SacII. At least two copies of Cfr42I

recognition site are required for efficient cleavage.