货品编号： Arbor Assays.K033-F1 品牌： Arbor Assays 品名： Catalase Fluorescent Activity kit (Two Plates) 规格：2 x 96 well plate

产品描述： The DetectX® Catalase Activity Kit is designed to quantitatively measure catalase activity in a variety of

samples. A bovine catalase standard is provided to generate a standard curve for the assay. Samples are

diluted in the provided Assay Buffer and added to the wells of a half area black plate. Hydrogen peroxide

is added to each well and the plate incubated at room temperature for 30 minutes. The supplied Fluorescent

detection Reagent is added, followed by diluted horseradish peroxidase and incubated at room temperature

for 15 minutes. The HRP reacts with the substrate in the presence of hydrogen peroxide to convert the

colorless substrate into a fluorescent product. The fluorescent product is read at 590 nm with excitation at 570 nm.

Increasing levels of catalase in the samples causes a decrease in H2O2 concentration and a reduction in

fluorescent product. The activity of the catalase in the sample are expressed in terms of units of catalase

activity per mL.