货品编号： thermo.21338

品牌： thermo

品名： EZ-Link Sulfo-NHS-LC-LC-Biotin

规格： 50mg

产品描述：

Thermo Scientific EZ-Link Sulfo-NHS-LC-LC-Biotin is a long-chain biotinylation reagent

for labeling antibodies, proteins and other molecules that have primary amines.

Features of EZ-Link Sulfo-NHS-LC-LC-Biotin:

• Protein labeling—biotinylate antibodies to facilitate immobilization, purification or detection

using streptavidin resins or probes

• Cell surface labeling—biotinylates only surface proteins of whole cells because the negatively

charged reagent does not permeate cell membranes

• Amine-reactive—reacts with primary amines (-NH2), such as the side-chain of lysines (K)

or the amino-termini of polypeptides

• Soluble—charged sulfo-NHS group increases reagent water solubility compared to ordinary

NHS-ester compounds

• Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved

• Doubly long —spacer arm (total length added to target) is 22.4 angstroms; this extended

arm helps to minimize steric hindrance for biotin binding

Sulfo-NHS-LC-LC-Biotin is the longest of three very similar EZ-Link Reagents that are water-soluble,

non-cleavable, and enable simple and efficient biotinylation of antibodies, proteins and any other

primary amine-containing macromolecules in solution. Specific labeling of cell surface proteins is

another common application for these uniquely water-soluble and membrane impermeable reagents.

Differing only in their spacer arm lengths, the three Sulfo-NHS-ester reagents offer the possibility of

optimizing labeling and detection experiments where steric hindrance of biotin binding is an important factor.

We manufacture biotin reagents to ensure the highest possible overall product integrity, consistency,

and performance for the intended research applications.

N-Hydroxysulfosuccinimide (NHS) esters of biotin are the most popular type of biotinylation reagent.

NHS-activated biotins react efficiently with primary amino groups (-NH2) in alkaline buffers to form

stable amide bonds. Proteins (e.g., antibodies) typically have several primary amines that are available as

targets for label