货品编号： Bioss.bs-0047R品牌： Bioss品名： Anti-Insulin Receptor alpha胰岛素受体α抗体规格： 100ul 研究领域：心血管 细胞生物 神经生物学 信号转导 生长因子和激素 激酶和磷酸酶 糖尿病 新陈代谢

抗体来源：Rabbit

克隆类型：Polyclonal

交叉反应：Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep,

产品应用：WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=0.2μg/Test IF=1:100-500 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量：80/152kDa

细胞定位：细胞膜

性 状：Liquid

浓 度：1mg/ml

免 疫 原：KLH conjugated synthetic peptide derived from human Insulin Receptor alpha:701-760/1382

亚 型：IgG

纯化方法：affinity purified by Protein A

储 存 液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

PubMed：PubMed

产品介绍：The human insulin receptor is a heterotetrameric membrane glycoprotein consisting of disulfide

linked subunits in a beta-alpha-alpha-beta configuration. The beta subunit (95 kDa) possesses a single

transmembrane domain, whereas the alpha subunit (135 kDa) is completely extracellular. The insulin

receptor exhibits receptor tyrosine kinase (RTK) activity. RTKs are single pass transmembrane receptors

that possess intrinsic cytoplasmic enzymatic activity, catalyzing the transfer of the gamma phosphate of

ATP to tyrosine residues in protein substrates. RTKs are essential components of signal transduction

pathways that affect cell proliferation, differentiation, migration and metabolism.

Included in this large protein family are the insulin receptor and the receptors for growth factors such as

epidermal growth factor, fibroblast growth factor and vascular endothelial growth factor. Receptor activation

occurs through ligand binding, which facilitates receptor dimerization and autophosphorylation of specific

tyrosine residues in the cytoplasmic portion. The interaction of insulin with the alpha subunit of the insulin

receptor activates the protein tyrosine kinase of the beta subunit, which then undergoes an autophosphorylation

that increases its tyrosine kinase activity. Three adapter proteins, IRS1, IRS2 and Shc, become phosphorylated

on tyrosine residues following insulin receptor activation. These three phosphorylated proteins then interact with

SH2 domain containing signaling proteins.