货品编号： Bioss.bs-5616R 品 牌： Bioss 品 名：Anti-Phospho-Smad3 (Ser425)磷酸化细胞信号转导分子SMAD3抗体 规 格： 100ul 产品类型 磷酸化抗体 研究领域 肿瘤细胞生物免疫学信号转导干细胞细胞凋亡生长因子和激素转录调节因子 抗体来源 Rabbit 克隆类型 Polyclonal 交叉反应 Human,Mouse,Rat,Chicken,Pig,Cow,Horse,Rabbit, 产品应用 WB=1:500-2000ELISA=1:500-1000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1μg/TestICC=1:100-500IF=1:100-500（石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. 分子量 47kDa 细胞定位 细胞核细胞浆细胞膜 性状 Liquid 浓度 1mg/ml 免疫原 KLH conjugated Synthesised phosphopeptide derived from human Smad3 around the phosphorylation site of Ser425:SV(p-S)-NH2 亚型 IgG 纯化方法 affinity purified by Protein A 储存液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. 保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. PubMed PubMed

产品介绍 Smad3 is a 50 kDa member of a family of proteins that act as key mediators of TGF beta superfamily signaling in cell proliferation,

differentiation and development. The Smad family is divided into three subclasses: receptor regulated Smads, activin/TGF beta receptor

regulated (Smad2 and 3) or BMP receptor regulated (Smad 1, 5, and 8); the common partner, (Smad4) that functions via its interaction to

the various Smads; and the inhibitory Smads, (Smad6 and 7). Activated Smad3 oligomerizes with Smad4 upon TGF beta stimulation and

translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. Phosphorylation of the two TGF beta

dependent serines 423 and 425 in the C terminus of Smad3 is critical for Smad3 transcriptional activity and TGF beta signaling.