货品编号Bioss.bs-10589R-100ul

品牌Bioss

品名Anti-Collagen IIⅡ型胶原蛋白抗体

规格100ul

抗体来源Rabbit

克隆类型Polyclonal

交叉反应Human, Mouse, Chicken, Dog, Pig, Cow, Horse, Rabbit,

产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量117kDa

细胞定位细胞外基质 分泌型蛋白

性 状Liquid

浓 度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from human Collagen II:231-330/1487

亚 型IgG

纯化方法affinity purified by Protein A

储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

PubMedPubMed

产品介绍Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y"

triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult

to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent

on NON DENATURED three dimensional epitopes. This may result in diminished reactivity of some antibodies

with denatured collagen or formalin fixed, paraffin embedded tissues. Type II collagen is a fibrillar collagen found

in cartilage and the vitreous humor of the eye. Collagen type II is essential for the normal embryonic development

of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. Mutations in this gene

are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer

Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia

Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C propeptide

of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene.