货品编号： Bioss.bsm-2006M 品 牌： Bioss 品 名：Anti-PCNA(1C11)增殖细胞核抗原单克隆抗体 规 格： 100ul 研究领域 肿瘤细胞生物免疫学细胞周期蛋白转录调节因子 抗体来源 Mouse 克隆类型 Monoclonal 克隆号 1C11 交叉反应 Human,Mouse,Rat,Chicken,Cow,Rabbit, 产品应用 WB=1:400-1000ELISA=1:500-1000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1ug/TestIF=1:100-500（石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. 分子量 29kDa 细胞定位 细胞核 性状 Liquid 浓度 1mg/ml 免疫原 KLH conjugated synthetic peptide derived from human PCNA:151-261/261 亚型 IgG 纯化方法 affinity purified by Protein G 储存液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. 保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. PubMed PubMed

产品介绍 Proliferating cell nuclear antigen (PCNA) is a 28kDa nuclear protein associated with the cell cycle, a nuclear protein vital for cellular DNA

synthesis. Proliferating cell nuclear antigen was originally identified by immunofluorescence as a nuclear protein whose appearance correlated

with the proliferate state of the cell. PCNA is required for replication of DNA in vitro and has been identified as the auxiliary protein (cofactor) for

DNA polymerase delta. The anti-PCNA antibodies react with the nuclei of proliferating cells. PCNA is essential for cellular DNA synthesis and is

also required for the in vitro replication of simian virus 40 (SV40) DNA where it acts to coordinate leading and lagging strand synthesis at the

replication fork. The PCNA protein may fulfil several separate roles in the cell nucleus associated with changes in its antigenic structure.