货品编号Bioss.bs-1634R

品牌Bioss

品名Anti-ATF6活化转录因子6抗体

规格100ul

抗体来源Rabbit

克隆类型Polyclonal

交叉反应Human, Mouse, Rat, Pig, Cow, Horse, Rabbit,

产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /test IF=1:100-500 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量75kDa

细胞定位细胞核

性 状Liquid

浓 度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from human ATF6:301-400/670

亚 型IgG

纯化方法affinity purified by Protein A

储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

PubMedPubMed

产品介绍ATF6 is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target

genes. It binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3')

and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. ATF6 could also be involved

in activation of transcription by the serum response factor. ATF6 exists as a homodimer and heterodimer with ATF6 beta.

The dimer interacts with the nuclear transcription factor Y (NF-Y) trimer through direct binding to NF-Y subunit C (NF-YC).

It also interacts with the transcription factors GTF2I, YY1 and SRF. Under ER stress the cleaved N-terminal cytoplasmic

domain translocates into the nucleus. The basic domain of ATF6 functions as a nuclear localization signal and the basic

leucine zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE. During the unfolded protein

response an approximately 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis.

The cleavage seems to be performed sequentially by site 1 and site 2 proteases. ATF6 is N glycosylated, phosphorylated in

vitro by MAPK14/P38MAPK and belongs to the bZIP family.