货品编号Bioss.bs-2007R

品牌Bioss

品名Anti-PCNA增殖细胞核抗原抗体

规格100ul

抗体来源Rabbit

克隆类型Polyclonal

交叉反应Human, Mouse, Rat, Chicken, Dog, Cow, Rabbit,

产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量29kDa

细胞定位细胞核

性 状Liquid

浓 度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from human PCNA:201-261/261

亚 型IgG

纯化方法affinity purified by Protein A

储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

PubMedPubMed

产品介绍Proliferation MarkerProliferating cell nuclear antigen (PCNA) is a 28kDa nuclear protein associated with the cell

cycle, a nuclear protein vital for cellular DNA synthesis. Proliferating cell nuclear antigen was originally identified

by immunofluorescence as a nuclear protein whose appearance correlated with the proliferate state of the cell.

PCNA is required for replication of DNA in vitro and has been identified as the auxiliary protein (cofactor) for DNA

polymerase delta. The anti-PCNA antibodies react with the nuclei of proliferating cells. PCNA is essential for cellular

DNA synthesis and is also required for the in vitro replication of simian virus 40 (SV40) DNA where it acts to coordinate

leading and lagging strand synthesis at the replication fork. The PCNA protein may fulfil several separate roles in the

cell nucleus associated with changes in its antigenic structure。