Catalog Number :BGK70460
MIP-3β is a CC chemokine, expressed in the thymus, lymph nodes, and in activated bone marrow stromal cells that signals through the CCR7 receptor. MIP-3β is a chemoattractant for T and B lymphocytes, and myeloid progenitor cells.
The BioGems Murine MIP-3β (CCL19) Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure MIP-3β (CCL19) with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for MIP-3β (CCL19). It contains E. coli-expressed recombinant MIP-3β (CCL19). The detection antibody is a biotinylated goat polyclonal antibody specific for MIP-3β (CCL19). The kit is analytically validated with ready to use reagents.
MIP-3β是一种CC趋化因子，在胸腺，淋巴结和活化的骨髓基质细胞中表达，通过CCR7受体传导信号。MIP-3β是T和B淋巴细胞和骨髓祖细胞的趋化因子（chemoattractant）。
BioGems鼠MIP-3β（CCL19）预包被ELISA（酶联免疫吸附测定）试剂盒采用固相免疫测定法，专门设计用于检测MIP-3β（CCL19），其中96孔微孔板预包被小鼠MIP-3β特异性单克隆抗体（CCL19）。 它含有大肠杆菌表达的重组MIP-3β（CCL19）。 检测抗体是对MIP-3β特异的生物素化山羊多克隆抗体（CCL19）。该试剂盒经过分析验证，是即用型试剂。
Product Details
|
Assay Type:
|
Solid Phase Sandwich ELISA: 96-Well Strip Plate
|
|
Sensitivity:
|
<10pg/ml
|
|
Range:
|
15.6pg/ml-1000pg/ml
|
|
Specificity:
|
Natural and recombinant Murine MIP-3β(CCL19)
|
|
Uniprot:
|
O70460
|
|
Interference:
|
No detectable cross-reactivity with other relevant proteins
|
|
Storage Conditions:
|
Store the unopened product at 2 – 8 ℃.
|
Components组份
|
Description
|
Quantity
|
Volume
|
|
Anti-Murine MIP-3β(CCL19) Pre-coated 96-well strip microplate
|
1
|
12 strips of 8 wells
|
|
Murine MIP-3β(CCL19) Standard
|
2
|
10 ng vial
|
|
Murine MIP-3β(CCL19) Biotinylated antibody (100x)
|
1
|
130 µL
|
|
Avidin-Biotin-Peroxidase Complex (100x)
|
1
|
130 µL
|
|
Sample Diluent
|
1
|
30mL
|
|
Antibody Diluent
|
1
|
12mL
|
|
Avidin-Biotin-Peroxidase Diluent
|
1
|
12mL
|
|
Wash Buffer Concentrate (Powder for 1000mL)
|
1
|
Pack
|
|
Color Developing Reagent
|
1
|
10mL
|
|
Stop Solution
|
1
|
10mL
|
|
Plate Sealers
|
4
|
Each
|
Reproducibility可重复性
|
|
Intra-Assay
|
Inter-Assay
|
|
Sample ID
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n=
|
16
|
16
|
16
|
24
|
24
|
24
|
|
Mean Measured Concentration (pg/mL)
|
91
|
286
|
592
|
105
|
379
|
645
|
|
Standard Deviation
|
3.82
|
14.3
|
34.93
|
6.51
|
28.05
|
52.25
|
|
Consistency (%CV)
|
4.2
|
5
|
5.9
|
6.2
|
7.4
|
8.1
|
©
金山科研平台 Thermo Fisher官网Nunc、QSP、Nalgene、Invitrogen、Gibco耗材试剂授权一级代理是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://thermonunc.cn/thread-19916.htm
特别声明：以上内容(如有图片亦包括在内)来源于授权厂家或网络，如有侵权，请联系客服删除，本平台不提供信息校正和存储服务。
Notice: The content above (including the pictures if any)comes from authorized manufacturers or networks. In case of infringement, please contact to delete it. This platform does not provide information storage services.
QQ:1749072012