货品编号： Invitrogen.450245

品牌：Invitrogen

品名：Zero Blunt™ TOPO™ PCR Cloning Kit, without competent cells

规格：25 reactions

产品描述

Zero Blunt TOPO PCR Cloning Kit provides a highly efficient, 5-minute, one-step cloning strategy ("TOPO Cloning") for the direct

insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector. Each kit has

the Zero Blunt TOPO vector (see figure) containing the ccdB gene for positive selection, only permitting growth of plasmid vectors

with recombinants. Zero Blunt TOPO kits are available with a variety of competent cells, or without competent cells, depending on

your needs and budget. Features of the Zero Blunt TOPO PCR Cloning Kits:

Fast and easy—go from PCR to clones in just 3 steps and in as little as 5 minutes hands-on time Efficient—obtain up to 95% clones with correct insert Proven—reliable performance for over a decade with over 4,000 citations Simple —no ligase, post-PCR procedures, or PCR primers containing specific sequences are required.

Zero Blunt TOPO Cloning Kits—overview

Vector: pCRBlunt II-TOPO vector—contains the ccdB gene, allowing for direct selection of recombinants

Cloning method: TOPO cloning—Topoisomerase I–based, 5-minute ligation of blunt-ended PCR products amplified with proofreading

thermostable polymerases

Competent cells: Various options—choose from kits with either general, high-efficiency, bacteriophage T1-resistant, fast-growing

competent cells, or use your own.

pCRBlunt II-TOPO vector—designed for proofreading polymerases The pCRBlunt II-TOPO vector is designed to clone blunt-ended PCR products generated by thermostable proofreading polymerases

such as Platinum Pfx DNA Polymerase. The pCRBlunt II-TOPO vector contains:

• EcoRI sites flanking the PCR product insertion site for easy excision of inserts • Kanamycin and Zeocin resistance genes for your choice of selection in E. coli • SP6 promoter/primer site for in vitro RNA transcription and sequencing • M13 forward and reverse primer sites for sequencing or PCR screening